Value of mNGS in sonication fluid for the diagnosis of periprosthetic joint infection

Arthroplasty

Table 3 Cases with discordant results between culture and mNGS

							Preoperative aspirate
Case	Age (y)/sex	Diagnosis	Antibiotic Prior to surgery	ESR	CRP	Volume/feature	Conventional Culture	Sonication Fluid Culture	Sonication mNGS	WBC (cells/μL)	PMN%
1	90/M	THA infection	Not used	17	17.1	10 ml/bloody	Negative	Staphylococcus aureus	Staphylococcus aureus	7838	88.2
2	63/F	THA infection	Not used	29	2.83	5 ml/bloody	Negative	Negative	Parvimonasmicra	7160	83.9
3	49/M	THA infection	Not used	47	11.5	8 ml/yellow-sticky	Negative	Negative	Mycoplasma hominis	6380	63.8
4	79/F	TKA infection	Not used	16	2.8	8 ml/bloody	Negative	Negative	Candida tropicalis	820	47.9
5	66/F	TKA infection	Used	8.07	65	8 ml/yellow-sticky	Negative	Negative	Staphylococcus epidermidis	9144	75.9
6	63/M	THA infection	Used	58	40.2	9 ml/bloody	Negative	Negative	Staphylococcus epidermidis	6870	83.4
7	63/M	THA infection	Used	58	40.2	15 ml/bloody	Negative	Negative	Staphylococcus epidermidis& Enterococcus faecalis	6870	83.4
8	63/M	TKA infection	Used	65	12.3	15 ml/yellow-sticky	Negative	Negative	Neisseria macacae&Candida parapsilosis	32925	90.3
9	89/M	TKA infection	Used	120	90	34 ml/purulent	Negative	Negative	Mycoplasma hominis	26286	85.6
10	74/M	TKA infection	Used	16	5.62	6 ml/purulent	Enterococcus faecalis	Enterococcus faecalis	Enterobacter cloacae	1717	62.7
11	58/F	TKA aseptic failure	Not used	27	8.43	10 ml/Clean	Negative	Staphylococcus epidermidis	Staphylococcus epidermidis	186	26.8

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